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. 2013 Dec 2;3:3388. doi: 10.1038/srep03388

Figure 5. cAMP may be involved in the inhibition of iNOS under ER stress.

Figure 5

(A) Glial cells were treated with tunicamycin (Tm: 1 μg/mL) for 1 h and then stimulated with dibutyryl cyclic AMP (dbcAMP: 1 mM) + IFNγ (20 ng/mL) for 24 h. iNOS protein levels were detected by Western blotting. dbcAMP + IFNγ-induced iNOS expression at the protein level was inhibited under ER stress. **p < 0.01 dbcAMP + IFNγ v.s. dbcAMP + IFNγ + Tm. n = 8. The GAPDH was analyzed as loading control. The intensities of the bands were expressed as iNOS/GAPDH. Full-length blots are presented in Supplementary Fig S6. (B) Glial cells were treated with tunicamycin (Tm: 1 μg/mL) for 1 h and then stimulated with dbcAMP (1 mM) + IFNγ (20 ng/mL) for 4 h. iNOS mRNA levels were detected by RT-PCR. dbcAMP + IFNγ-induced iNOS expression at the mRNA level was not inhibited under ER stress. n = 5. The GAPDH was analyzed as loading control. Full-length gels are presented in Supplementary Fig S4. Cropped gels/blots were run under the same experimental conditions.