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. 2013 Oct 11;32(23):3029–3040. doi: 10.1038/emboj.2013.223

Figure 2.

Figure 2

The stress-responsive kinase p38 is required for dispersal of centriolar satellite factors in response to a range of cell stresses. (A) U2OS cells were exposed to UV, heat shock, or the transcriptional inhibitors DRB or Actinomycin D, fixed and immunostained with AZI1 antibody. Scale bar, 10 μm. (B) U2OS cells were incubated with p38 inhibitor (p38i) for 1 h or transfected with p38 siRNA for 72 h. Subsequently, cells were irradiated or not with UV, fixed 1 h later and stained with AZI1 antibody. Scale bar, 10 μm. (C) U2OS cells treated as in (B) were stained with AZI1 and γ-tubulin antibodies. Scale bar, 10 μm. (D) U2OS cells treated as in (B) were immunoblotted with indicated antibodies. (E) Cytoskeletal and cytoplasmic fractions of cells treated with p38 inhibitor and/or UV for 1 h prior to harvest were analysed with the indicated antibodies. (F) U2OS cells transfected with WT or kinase-dead (K82A) versions of FLAG-tagged MKK6 were fixed and immunostained with AZI1 and FLAG antibodies. Scale bars, 10 μm.