Table 1.
PCR primers and conditions.
| Product | Amplicon Size |
Forward Primer | Reverse Primer | Annealing Temperature |
|---|---|---|---|---|
| Exon 2 | 398bp | 5’-GCCTCTTGGCCCTCTGGCAA-3’ | 5’-TGCCCACATTCCCAGGGTGAGA-3’ | 63°C |
| Exon 4 | 575bp | 5’-CTCAGAGCCTTGGCTGGGTGTA-3’ | 5’-CCCGCATCTGCCTCCCCAATG-3’ | 63°C |
| Full codon |
706bp | 5’-GGCTCTTTGCGTCTGCGTAGTT-3’ | 5’-CCCGCATCTGCCTCCCCAATG-3’ | 63°C |