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. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: Cancer Res. 2008 Oct 1;68(19):10.1158/0008-5472.CAN-07-6538. doi: 10.1158/0008-5472.CAN-07-6538

Figure 3.

Figure 3

p53-dependent release of exosomes from prematurely senescent human prostate cancer cell lines. (a) Parental 22Rv1 cells and derivatives expressing a dominant-negative p53 mutant (DN p53) were exposed to either 0 Gy or 4 Gy and on day 4 after irradiation (Left panel) the percentage of SA β-Gal-positive cells were determined as in Figure 2a or (Right panel) or cells were used to condition serum-free medium for 48 h. Exosome fractions were isolated from the conditioned medium and stained using Vybrant DiI. Fluorescence intensity was normalizing for differences in cell survival and data represent the means (± s.e.) of two independent experiments done in triplicate. (b) Results of experiments conducted using LNCaP cells under identical conditions to those described in (a), above.