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Epac1 inhibition blocks rickettsial invasion by impeding the expression of Ku70 outside of the nucleus in HUVECs. Representative dual-target IF staining of rickettsiae (red) and Ku70 (green) in ESI-09– and vehicle-exposed HUVECs 30 min postinfection with R. australis. Nuclei of HUVECs were counterstained with DAPI (blue). (Scale bars: 10 µm.)
