Fig. 2.
Foretinib and crizotinib inhibit FIG–ROS and effector signaling in murine cholangiocarcinoma tumor cell lines. (A) IC50 values for shPten- (lines 1 and 2) and FIG–ROS (lines 3 and 4)-driven murine cholangiocarcinoma cell lines treated with indicated inhibitors as determined from nonlinear regression curve fit analysis of the dose–response curves. (B) Analysis of anchorage-independent colony formation of FIG–ROS (line 4) and shPten (line 2) cholangiocarcinoma cell lines treated with inhibitors at the indicated concentrations. The numbers of colonies formed in the presence of foretinib are shown as a percentage of the number of colonies with vehicle treatment. Colony number is an average obtained using three independent wells normalized to vehicle control. (C) Immunoblot analysis of FIG–ROS fusion protein phosphorylation and downstream effector modulation after varying doses of crizotinib and foretinib. Cropped images representative of three independent experiments are shown.