Fig. 1.

Sequential FISH- and GISH-based karyotypes in the four synthetic allotetraploid wheats and their diploid parental species (SI Materials and Methods). The boxed chromosomes are those showing localized, persistent genomic changes in the synthetic tetraploid wheats relative to their diploid parents. A, B, C, and D respectively represent FISH and GISH images of AT1, AT2, AT3, and AT4 along with their corresponding diploid parental species. The probes used in FISH are pSc119.2 (green) and pAS1 (red). (Scale bars, 10 μm.)