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. 2013 Nov 11;110(48):19438–19443. doi: 10.1073/pnas.1315075110

Fig. 3.

Fig. 3.

Early limb patterning pathways are not disrupted, but posterior HoxD gene expression is anteriorized in Hox5 mutants. (A–J) Early AP patterning factors are not disrupted in Hox5 mutants. The expression of Gli3 is not altered at E9.5 (A and F) or E10.5 (C and H). Hand2 is expressed normally in Hox5 mutant forelimbs at E9.5 (B and G) and E10.5 (D and I). Alx4 expression in Hox5 mutants is also comparable to that in controls at E10.5 (E and J). (K–R) The expression limits of posterior HoxD genes are anteriorized in E10.5 Hox5 mutant forelimbs. Hoxd10 (K and O), Hoxd11 (L and P), Hoxd12 (M and Q), and Hoxd13 (N and R) are all expressed more anteriorly in Hox5 mutant forelimb buds (O–R) compared with controls (K–N). Black arrowheads mark the WT anterior boundary of expression for each probe.