Skip to main content
. 2013 Oct 23;110(48):E4571–E4580. doi: 10.1073/pnas.1311669110

Fig. 6.

Fig. 6.

IFI16 is essential for control of HIV-1 infection in primary human macrophages. (A) Levels of HIV-1 Gag DNA in hMDMs infected with HIV-1 Bal for 12 h were measured by qPCR (n = 2). (B) Summary of experimental setup in hMDMs. Primary monocytes were differentiated into macrophages and treated with siRNA (165 nM) before HIV-1 Bal infection (MOI of 1 calculated on Tzmbl titration and X-Gal straining). (C) Levels of IFI16 knockdown by a scramble and specific siRNA were measured by RT-qPCR on RNA harvested on the day of viral infection. Results represent four independent siRNA transfections made in cells from both donors (mean ± SEM). (D and E) hMDMs from donors A (D) and B (E) treated with control or IFI16 siRNA were infected with HIV-1 Bal (MOI of 1.0). Cultures were harvested at the indicated time points, and p24 levels were measured by ELISA (n = 4; two-way ANOVA with Bonferroni multiple comparison). (F) Representative images of hMDMs from donor A at day 4 after viral infection. Arrows indicate cells exhibiting apoptotic morphology.