Figure 3.

Effect of 7,3′,4′-THIF on clinical severity in NC/Nga mice. (a) The schedule of animal experiments. (b) Effect of 7,3′,4′-THIF on DFE-induced AD in NC/Nga mice. (A) Untreated control group; (B) DFE-treated group; (C) DFE plus low-dose 7,3′,4′-THIF (4 mg/kg); (D) DFE plus high-dose 7,3′,4′-THIF (20 mg/kg). (c) Effect of 7,3′,4′-THIF on scratching incidence. (d) Effect of 7,3′,4′-THIF on ear thickness. (e) Effect of 7,3′,4′-THIF on TEWL. Scratching behavior, ear thickness, and TEWL were measured 1 week before mice were euthanized. Data represent means ± SEM (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with DFE-treated mice.