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. 2013 Dec 2;4:407. doi: 10.3389/fimmu.2013.00407

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Copyright © 2013 Pérez, Romeu, Cabrera, González, Batista-Duharte, Labrada, Pérez, Reyes, Ramírez, Sifontes, Fernández and Lastre.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Passive cutaneous anaphylaxis. Male rats were inoculated subcutaneously with 100 μL of sera from mice immunized intramuscularly with two doses 15 days apart of Dermatophagoides siboney (Ds) allergen adsorbed onto aluminum hydroxide (Alum) at 1 or 5 μg per dose, or adjuvanted with AFPL1 (PL at 12 μg) in Alum at Ds concentrations of 0.5–2.5 μg per dose. Controls included AFPL1 or Alum alone. Sera were prepared at 0, 15, and 35 days. After 48 h, Evans Blue plus 1 mg of Ds was inoculated intravenously. Then the rats were sacrificed and the skin was used to evaluate the density and diameter of the stain. (A) Shows the evaluation of undiluted sera and (B) the quantity of the specific IgE dilution in the sera.