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. 2013 Dec 2;4:407. doi: 10.3389/fimmu.2013.00407

Figure 7.

Figure 7

Th1 cytokine profile and antibody affinity maturation after a plain PsA booster. Groups of mice received 5 μg PsA adsorbed onto Alum or together with 5 μg of AFPL1 or conjugate vaccine MenAfriVac® via the subcutaneous route. Immunizations were performed twice, 21 days apart. A subcutaneous booster of plain PsA was performed 105 days after the last dose. Spleens from individual mice after the booster immunization were pooled within each group. (A) Cytokine levels, measured by ELISA are expressed as pg/mL ± SEM. (B) affinity index (AI) was determined by avidity ELISA with 250 mM sodium thiocyanate. The AI, percentage of antibodies that remain bound to the antigen after treatment with the chaotropic agent, was calculated using the following formula: AI = titer (NaSCN+)/titer (NaSCN−). Results are expressed as the geometric mean of the affinity index (%). Data were analyzed by one-way ANOVA followed by a Tukey’s multiple comparison test. A p-value of <0.05 was considered statistically significant and is represented by different letters.