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. Author manuscript; available in PMC: 2013 Dec 2.

Published in final edited form as: J Immunol. 2009 Mar 15;182(6):10.4049/jimmunol.0803121. doi: 10.4049/jimmunol.0803121

Immature anti-insulin B cells chronically cultured with insulin in vitro show impaired proliferation to anti-CD40. Bone marrow culture-derived (Fig. 2B) 125Tg (A) or IgHELMD4Tg (B) B cells were naive (□) or cultured with human insulin (500 ng/ml, , or 5 × 10⁴ ng/ml, ■) for 1 day, then stimulated with anti-CD40 (x-axis) with or without insulin indicated. [³H]Thymidine incorporation (cpm) was measured. The average ± SD of triplicate determinations of three animals is shown; *, p < 0.03 and **, p < 0.007, as calculated by a two-tailed, two-sample/unequal variance t test.

Inline graphic — Immature anti-insulin B cells chronically cultured with insulin in vitro show impaired proliferation to anti-CD40. Bone marrow culture-derived (Fig. 2B) 125Tg (A) or IgHELMD4Tg (B) B cells were naive (□) or cultured with human insulin (500 ng/ml, , or 5 × 10⁴ ng/ml, ■) for 1 day, then stimulated with anti-CD40 (x-axis) with or without insulin indicated. [³H]Thymidine incorporation (cpm) was measured. The average ± SD of triplicate determinations of three animals is shown; *, p < 0.03 and **, p < 0.007, as calculated by a two-tailed, two-sample/unequal variance t test.