Figure 3.
Flagellin-mediated potentiation of TCR-induced cytokine production in HuT78 T cells is transient. (A) HuT78 T cells were treated without flagellin (0 hour timepoint) or with 250 ng/ml flagellin for various times. The cells were washed and then stimulated with 1μg/ml plate-bound anti-CD3 (OKT3) overnight. (B) HuT78 T cells pretreated with or without 250 ng/ml flagellin and incubated overnight. The cells were washed, rested for various times, and then stimulated with 1μg/ml plate-bound anti-CD3 for 24 hours. The supernatants were assayed by ELISA for IL-2 production. The results are shown as the average ± SEM of at least three independent experiments normalized to the maximal IL-2 production in the individual experiment. The data was statistically compared (* = p<0.05 and ** = p<0.01) using ANOVA with Dunnett's Multiple Comparison Post Test with the reference treatment marked with #.