Table 2.
The List of 22 Paralog Groups Identified in This Study and the Numbers of Codon-Based Substitutions per Site Calculated for Each Group
| Paralog Group | Na | Ave. dS (SE) | Ave. dN (SE) | Ave. dN/dS | Pb | dN/dS > 1c |
|---|---|---|---|---|---|---|
| Rn-RNase1 | 3 | 0.122 (0.023) | 0.113 (0.015) | 0.939 | NS | 1/3 |
| Cp-RNase1 | 3 | 0.174 (0.027) | 0.128 (0.017) | 0.750 | NS | 0/3 |
| Bt-RNase1 | 3 | 0.204 (0.028) | 0.104 (0.016) | 0.514 | 1.00 × 10−4 | 0/3 |
| Ml-RNase1 | 7 | 0.055 (0.012) | 0.056 (0.008) | 0.997 | NS | 12/21 |
| Mm-EAR | 7 | 0.144 (0.018) | 0.189 (0.016) | 1.310 | 0.0151 | 18/21 |
| Rn-EAR | 5 | 0.141 (0.021) | 0.125 (0.015) | 0.383 | NS | 2/10 |
| Oc-RNase2/3 | 3 | 0.287 (0.030) | 0.163 (0.018) | 0.568 | NS | 0/3 |
| Ml-RNase2/3 | 2 | 0.093 (0.025) | 0.066 (0.015) | 0.710 | — | 0/1 |
| Cp-RNase4d | 2 | 0 | 0.003 (0.003) | ∞ | — | 0/1 |
| Ml-RNase4 | 11 | 0.125 (0.016) | 0.160 (0.015) | 1.343 | 1.45 × 10−6 | 44/55 |
| Mm-RNase5 | 5 | 0.136 (0.020) | 0.145 (0.015) | 1.093 | NS | 5/10 |
| Rn-RNase5 | 2 | 0.043 (0.017) | 0.014 (0.007) | 0.326 | — | 0/1 |
| Bt-RNase5 | 3 | 0.220 (0.029) | 0.210 (0.021) | 0.949 | NS | 0/3 |
| Ml-RNase5 | 7 | 0.053 (0.012) | 0.091 (0.013) | 2.002 | 1.21 × 10−7 | 20/21 |
| Hg-RNase6 | 4 | 0.308 (0.028) | 0.303 (0.023) | 0.979 | NS | 3/6 |
| Cp-RNase6 | 3 | 0.435 (0.035) | 0.343 (0.025) | 0.783 | NS | 0/3 |
| Am-RNase6 | 5 | 0.220 (0.024) | 0.227 (0.017) | 1.048 | NS | 5/10 |
| La-RNase6 | 3 | 0.127 (0.025) | 0.124 (0.018) | 0.985 | NS | 1/3 |
| Oc-RNase7/8 | 2 | 0.271 (0.041) | 0.146 (0.022) | 0.539 | — | 0/1 |
| Bt-RNase7/8 | 2 | 0.135 (0.029) | 0.107 (0.019) | 0.796 | — | 0/1 |
| Mmu-RNase9 | 2 | 0.019 (0.011) | 0.040 (0.010) | 2.105 | — | 1/1 |
| Bt-RNase9 | 2 | 0.242 (0.036) | 0.222 (0.023) | 0.917 | — | 0/1 |
Note.—Average dS (numbers of synonymous substitutions per synonymous sites), dN (numbers of nonsynonymous substitutions per nonsynonymous sites), and dN/dS values of all possible paralog pairs for each group were measured. Standard errors (SE) are shown in parentheses. NS, not significant. P value higher than 0.05. —: Not applicable. We did not perform statistical tests for groups with only two paralogs (i.e., one paralog pair). The paralog groups with average dN/dS value higher than one with statistical significance are bolded.
aNumber of functional paralogs in each group.
bP values of the one-tail t-test for difference between dN and dS, only applied to groups of three or more paralogs.
cNumber of paralog pairs with dN/dS > 1 out of the total number of paralog pairs.
dThe paralog group of Cp-RNase4 has two recently duplicated genes different by only one nucleotide that also cause an amino acid difference.