Fig. 4.—

Positive selection within the RING domain of TRIM52. (A) More than half the sites predicted to be evolving under positive selection (fig. 1 and table 1) are located within the RING domain of TRIM52. To further highlight this, we identified the number of synonymous (S) and nonsynonymous (N) substitutions that have occurred in the expanded loop 2 region of TRIM52 in primate evolution (the equivalent domain of TRIM41 is shown for comparison). Examples of dramatic episodes of lineage-specific positive selection in TRIM52’s RING domain are highlighted in bold. (B) The differences in evolutionary signals are further demonstrated by an alignment of a 90 amino acid-long stretch of the loop 2 region from primate TRIM41 and TRIM52. Sites of positive selection are highlighted with a star and boldface.