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. Author manuscript; available in PMC: 2014 Nov 1.

Published in final edited form as: Dev Biol. 2013 Sep 10;383(1):10.1016/j.ydbio.2013.09.006. doi: 10.1016/j.ydbio.2013.09.006

Fig. 1 — A–D. A luciferase reporter plasmid containing the Myf5 ES enhancer and minimal promoter was transiently transfected into 3T3 cells with or without expression plasmids for Zic1, Zic2, Gli1, Gli2, Pax3, or Pax7. Cells were harvested 48 h post-transfection and assayed for luciferase activity. Data are plotted as the mean value and standard deviation of relative luciferase activity, with activity of the reporter construct alone set at 1. Pax3 synergizes with Zic1 (A, asterisk = p < 0.01) and Gli2 (B, asterisk = p < 0.05) in transactivating the reporter (one-tailed, one-step t-test comparing activity of factors in combination to the sum of individual factors), whereas other combinations of factors show no synergistic effects (C-D). E. 10T1/2 cells were transfected with Pax3-HA and FLAG-Zic1 expression plasmids, and cell extracts were immunoprecipitated with antibodies to FLAG, Gli2, or V5 (as a negative control). Immunoprecipitates were subjected to Western analysis using HA antibodies.

Fig. 1 — A–D. A luciferase reporter plasmid containing the Myf5 ES enhancer and minimal promoter was transiently transfected into 3T3 cells with or without expression plasmids for Zic1, Zic2, Gli1, Gli2, Pax3, or Pax7. Cells were harvested 48 h post-transfection and assayed for luciferase activity. Data are plotted as the mean value and standard deviation of relative luciferase activity, with activity of the reporter construct alone set at 1. Pax3 synergizes with Zic1 (A, asterisk = p < 0.01) and Gli2 (B, asterisk = p < 0.05) in transactivating the reporter (one-tailed, one-step t-test comparing activity of factors in combination to the sum of individual factors), whereas other combinations of factors show no synergistic effects (C-D). E. 10T1/2 cells were transfected with Pax3-HA and FLAG-Zic1 expression plasmids, and cell extracts were immunoprecipitated with antibodies to FLAG, Gli2, or V5 (as a negative control). Immunoprecipitates were subjected to Western analysis using HA antibodies.