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. 2013 Dec 2;7:231. doi: 10.3389/fncel.2013.00231

Figure 2.

Figure 2

SLM-generated 3D photostimulation pattern with DMD gating. (A) Nine-foci light pattern produced by the SLM with all DMD pixels turned ON showing zero order (0 order), second order [+2 (A)] and mirror projections [−1 (A–C)] of the first diffraction order [+1 (A–C)]. (B) Same photostimulation pattern as in (A) but with only the nine desired foci gated by the DMD. All other light is eliminated by being directed to the beam dump. (C) Power on one ON focus as a function of number of foci, N (n = 10 different spot configurations). For N =2–20, the ON focus was kept at a fixed position. Solid line is 1/N fit. (D) Normalized power as a function of DMD array size (n = 10 focal spot positions). Solid line is a cumulative Gaussian fit. (E,F) Peak of glutamate-evoked voltage from one ON uncaging site as a function of (E) number of uncaging sites and (F) DMD array size (n = 8–10 trials). Solid lines are proportional to squares of curve fits in C and D, respectively.