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Optimized concentrations for qPCR and dPCR assays.
| Component | qPCR | dPCR | ||
|---|---|---|---|---|
| Kefeng6 (μL) | gos9 (μL) | Kefeng6 (μL) | gos9 (μL) | |
| TaqMan Universal Master Mix (2×) | 12.5 | 12.5 | 5 | 5 |
| Primer-F (10 µM) | 1 | 1 | 0.4 | 0.4 |
| Primer-R (10 µM) | 1 | 1 | 0.4 | 0.4 |
| Probe-P (10 µM) | 0.5 | 0.5 | 0.2 | 0.2 |
| Template (~20 ng/μL) | 5 | 5 | 2 | 2 |
| Loading (10×) | 0 | 0 | 1 | 1 |
| H2O | 5 | 5 | 1 | 1 |
|
| ||||
| Total | 25 | 25 | 10 | 10 |
