Table 3.
Copy number concentrations and ratios of the third sample (S3) as determined by dPCR.
| Kefeng6 | gos9 | Concentration | Ratio | P | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive partitions | Estimated copies | Average Number | RSD (%) | Positive partitions | Estimated copies | Average number | RSD (%) | Mean (cp/µL) | RSD (%) | KF6/gos9 | |
| 494 | 786 | 794 | 2.6 | 476 | 774 | 790 | 3.3 | 792 | 2.87 | 1.005 | 0.786 |
| 471 | 780 | 494 | 821 | ||||||||
| 516 | 823 | 492 | 784 | ||||||||
| 465 | 797 | 455 | 769 | ||||||||
| 481 | 811 | 446 | 767 | ||||||||
| 455 | 766 | 488 | 825 | ||||||||
The average number was calculated from six replicate measurements with three parallel panels per chip (n = 6), and this value was adjusted to account for the gravimetrically prepared PCR solutions used in the Kefeng6 and gos9 assays. RSD: relative standard deviation; P: probability.