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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Sci Signal. 2013 Sep 24;6(294):10.1126/scisignal.2004379. doi: 10.1126/scisignal.2004379

Fig. 1. Abundance and phosphorylation status of receptor tyrosine kinases reveals heterogeneity in receptors within clinical subtypes in 39 breast cancer cell lines.

Fig. 1

(A) Bars indicate the abundance of the receptor tyrosine kinases, colored by clinical subtypes (red, TNBC or nonmalignant; yellow, HER2amp; purple, HR+). The black line is the amount of phosphorylated receptor. Dashed lines show the detection threshold for total protein abundance (grey) and phosphoprotein abundance (black). (B) Abundance of the estrogen and progesterone receptors (ER, solid; PR dashed; sum of ER and PR as bars). Horizontal gray lines show the detection thresholds (ER, solid; PR dashed). (C) Clustering of cell lines according to their basal profile defines three main clusters (BI, blue; BII, green; BIII, yellow; outliers, black). Color intensity in the heat map indicates abundance of the indicated protein or phosphoprotein in pg/cell for protein abundance and arbitrary units for phosphorylation measurements (measurements below detection threshold are in gray). Black horizontal lines show the cluster separations. (D) Projection of cell lines on the first two principal axes of a PCA (fig. S3) of the basal measurements. (E) Projection of cell lines on the indicated key variables identified by PCA. Dashed lines show the detection thresholds for each measurement.