Flow cytometry. (a) Flow cytometry 24 h after treatment; (b) flow cytometry 48 h after treatment; (c) histogram of flow cytometry 24 h after treatment; and (d) histogram of flow cytometry 48 h after treatment. Control: AGS cells treated with saline; 5-Fu: AGS cells treated with 0.5 μg/mL 5-Fu; TRAIL: AGS cells treated with 2 μg/mL TRAIL; and combined: AGS cells treated with 0.5 μg/mL 5-Fu and 2 μg/mL TRAIL. The apoptosis rates were 3.8 ± 0.7%, 9.1 ± 1.1%, 11.6 ± 0.7%, and 19.65 ± 6.45%, respectively, in the control, 5-Fu, TRAIL, and combined groups 24 h after treatment and were 4.63 ± 0.47%, 8.55 ± 0.65%, 11.96 ± 1.12%, and 17.27 ± 1.67%, respectively, in the control, 5-Fu, TRAIL, and combined groups 48 h after treatment. &
P < 0.05 compared with control and 5-Fu groups, #
P < 0.05 compared with control, 5-Fu, and TRAIL groups, and ▲
P < 0.01 compared with control, 5-Fu, and TRAIL groups.