Fig. 3.

Tpl2 ^+/+ primary keratinocytes migrate faster than Tpl2 ^−/− primary keratinocytes in the presence of mitomycin C. (A) Scratch test assay in v-ras^Ha retrovirus-infected Tpl2 ^+/+ and Tpl2 ^−/− primary keratinocytes treated with (left) or without (right) 10 μg/ml mitomycin C. Cells were photographed every 4h and closure calculated using IncuCyte software. Error bars were calculated using standard deviation from quadruplicate samples. Significance at P < 0.05 was determined through Student’s t-tests. (B) Scratch test assay in v-ras^Ha retrovirus-infected keratinocytes. Tpl2 ^+/+ keratinocytes (left panel) and Tpl2 ^−/− keratinocytes (right panel) were scratched, treated with 10 μg/ml mitomycin C and visualized using IncuCyte Live Cell Imaging System at ×10 magnification.