Figure 5. Differentiation assays of VEE-RF RNA iPS Cell Clones.
(A) VEE-RF RNA iPS clones were differentiated into cardiomyocytes as described in Experimental Procedures. Contractile embryoid bodies (EBs) were recorded (see supplemental movie S1), and then dissociated and replated onto slides for immunofluorescence staining with mouse anti-Cardiac Troponin or mouse anti-alpha-Actinin, Anti-Mouse IgG Alexa Fluor 488, and DAPI. Bar, 50 μm.
(B) Teratoma formation of VEE-RF RNA iPS clones in nude mice. H&E staining; Bar, 100 μm.
(C) Immunohistochemistry staining of VEE-RF RNA iPS cell clone teratomas in nude mice. AE1/AE3 (cytokeratin), NF-1 (neuronal cells) and GFAP (neuronal cells) used for markers of ectoderm; Desmin (muscle cells) used for marker of mesoderm; and AFP (primitive and definitive endoderm) used for marker of endoderm. Bar, 100 μm.