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NS5A co-immunoprecipitates with HSP40 and HSP70. Western analysis of NS5A-Flag immunoprecipitation probed for HSP40 and HSP70 demonstrates NS5A/HSP40 and NS5A/HSP70 complex formation. Input is shown at a lower exposure because of the abundance of HSP40 and HSP70 in these extracts. C, control GFP; N, NS5AFLAG. N = 2 with reproducible results.
