Table 2. Evaluation of analgesic activity by Hot plate method.
| Treatment | Average Reaction Time in seconds before treatment (Mean ± S.E.M.) | Reaction time in seconds after treatment (Mean ± S.E.M.) | %MPE | ||
| 15 min | 30 min | 60min | |||
| Control | 4.75±0.1547 | 4.75±0.1583 | 4.75±0.2563 | 4.75±0.4941 | - |
| Pentazocine | 4.70±0.4012** | 7.70±0.5211** | 9.67±0.4302** | 11.81±0.3254** | 69.02 |
| IIIa | 4.56±0.5221** | 7.23±0.6326** | 9.23±0.5745** | 10.76±0.4369** | 59.38 |
| IIIb | 4.63±0.4785** | 7.49±0.2234** | 9.87±0.3865** | 11.15±0.5356** | 62.87 |
| IIIc | 4.67±0.4523** | 6.52±0.6542** | 9.34±0.5413** | 9.53±0.4356** | 47.04 |
| IIId | 4.63±0.6374** | 7.46±0.7585** | 9.52±0.2658** | 10.47±0.7541** | 56.31 |
| IIIe | 4.69±0.4474** | 6.45±0.5428** | 8.36±0.6756** | 9.27 ±0.5854** | 44.42 |
| IIIf | 4.65±0.4469** | 7.42±0.3769** | 9.49±0.5369** | 10.71±0.5775** | 58.55 |
| IIIg | 4.56±0.5854** | 6.70±0.4459** | 8.49±0.7474** | 9.27±0.6525** | 45.11 |
| IIIh | 4.43±0.3544** | 7.50±0.7585** | 9.57±0.6456** | 10.52 ±0.6785** | 57.61 |
| IIIi | 4.71±0.4675** | 6.39±0.3646** | 9.03±0.5236** | 9.46±0.5359** | 46.16 |
| IIIj | 4.68±0.7548** | 7.56±0.5636** | 9.52±0.6552** | 11.37±0.6426** | 64.82 |
| IVa | 4.72±0.5957** | 7.68±0.4523** | 9.56±0.3356** | 11.09±0.5517** | 61.96 |
| IVb | 4.67±0.4358** | 7.45±0.6984** | 9.16±0.3548** | 10.59±0.5478** | 57.30 |
| IVc | 4.55±0.4785** | 6.59±0.4578** | 9.44±0.5349** | 9.69±0.8689** | 49.18 |
| IVd | 4.58±0.5878** | 7.66±0.6689** | 9.50±0.4245** | 11.56±0.6741** | 66.98 |
| IVe | 4.39±0.4589** | 6.89±0.7481** | 8.59±0.6478** | 9.75 ±0.4589** | 50.51 |
| IVf | 4.66±0.5958** | 7.53±0.3549** | 9.80±0.3358** | 11.61±0.6358** | 67.21 |
| IVg | 4.45±0.5869** | 7.72±0.5269** | 9.58±0.5895** | 10.46±0.5692** | 56.96 |
| IVh | 4.41±0.5321** | 7.80±0.4781** | 9.57±0.5696** | 10.82 ±0.3324** | 60.52 |
| IVi | 4.64±0.2966** | 7.56±0.3826** | 9.45±0.7851** | 10.89±0.5369** | 60.32 |
| IVj | 4.52±0.2853** | 7.70±0.5239** | 9.43±0.4665** | 10.44±0.6745 ** | 56.48 |
| IV3a | 4.64±0.4789** | 7.34±0.3545** | 9.12±0.3456** | 10.12±0.2985** | 52.89 |
| IV3b | 4.56±0.5884** | 7.11±0.5789** | 8.36±0.6398** | 9.47±0.6489** | 47.03 |
| IV3c | 4.67±0.3956** | 7.49±0.5212** | 9.27±0.3369** | 10.76±0.6245** | 58.95 |
| IV3d | 4.73±0.4525** | 7.13±0.2845** | 9.10±0.3235** | 9.46±0.5469** | 46.05 |
| IV3e | 4.59±0.5823** | 6.93±0.5861** | 9.14±0.6478** | 10.74 ±0.4369** | 59.07 |
| IV3f | 4.54±0.4969** | 7.23±0.5326** | 9.50±0.3756** | 10.32±0.6542** | 55.25 |
| IV3g | 4.49±0.5478** | 7.02±0.4689** | 8.48±0.5225** | 8.93±0.5692** | 42.24 |
| IV3h | 4.61±0.4526** | 6.80±0.4369** | 8.55±0.5236** | 9.51 ±0.5236** | 47.16 |
| IV3i | 4.67±0.6325** | 6.56±0.3989** | 8.47±0.5364** | 9.37±0.6346** | 45.49 |
| IV3j | 4.71±0.5963** | 7.31±0.2845** | 9.46±0.3623** | 10.20±0.4126 ** | 53.35 |
| Ih | 4.46±0.6522** | 7.25±0.4545** | 8.55±0.2625** | 9.88±0.4121** | 51.18 |
| Ii | 4.29±0.3532** | 7.45±0.4666** | 9.12±0.3644** | 9.83±0.5481** | 51.72 |
| Ij | 4.34±0.5418** | 7.75±0.6641** | 9.85±0.4236** | 10.24±0.3678** | 55.34 |
| IIh | 4.54±0.6645** | 6.96±0.4425** | 8.86±0.3356** | 9.87±0.4356** | 50.95 |
| Iii | 4.59±0.5414** | 6.45±0.3784** | 8.22±0.3541** | 9.45±0.4678** | 46.68 |
| IIj | 4.74±0.4775** | 7.35±0.4125** | 9.45±0.5925** | 10.96±0.6486** | 60.62 |
| Dose: 25 mg/kg for compounds Ih-Ij, 50mg/kg for IIIa-j and IVa-j, 100mg/kg for IV3a-j and 5mg/Kg for Pentazocine. ** p<0.01 represent the significant difference when compared control group. | |||||