Figure 4.

Electrophoretic pattern of purified TNF-α with CNBr-activated sepharose 4B beads. Expressed Raji cells lysates was dialyzed against PBS buffer, pH 7.4 and loaded on column containing coupled purified scFv-CNBr-activated sepharose 4B beads. The purity of eluted protein was analyzed by SDS-PAGE which was up to 95%. The major band at a molecular weight of approximately 17 kDa related to TNF-α protein was observed. Lane 1: Crude protein extract before chromatography, Lane 2: Pure elution fraction. Lane 3: Protein size marker. The gel was stained with Coomassie Blue R-25.