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. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: Anticancer Res. 2010 Sep;30(9):3333–3339.

Figure 2.

Figure 2

Treatment with XN induces apoptosis in prostate cancer cells. A: XN increases annexin V-FITC binding. C4-2 and PC-3 cells were treated with XN at concentrations of 5 to 40 μM for 72 h. Cells were then reacted with 5 μl of annexin V-FITC reagent for 30 min at room temperature. The percentage of annexin V-FITC-positive tumor cells was determined by flow cytometry. Results are presented as percentage of annexin V-FITC-binding cells. B: XN cleaves PARP-1. Cells were treated with XN as described above and cleavage of PARP-1 was analyzed by immunoblotting. C: XN cleaves procaspases -3, -8 and -9. C4-2 and PC-3 cells were treated with XN at concentrations of 5 to 40 μM 72 h and cleavage of procaspases-3, -8 and -9 was probed by Western blotting. Each experiment was repeated at least two times.