Figure 4.
XN inhibits prosurvival p-Akt, NF-κB and p-mTOR and antiapoptotic Bcl-2, Bcl-xL and survivin. C2-4 and PC-3 cells were treated with XN at 0 to 40 μM for 72 h. After treatment, cell lysates were prepared and analyzed by Western blotting using anti-p-Akt antibody, anti-NF-κB (p65), anti-p-mTOR antibodies (A) or anti-Bcl-2, ant-Bcl-xL, anti-survivin antibody (B) or anti-β-actin antibody (loading control).