Fig. 3.

P2X7 inhibition induces counteracting growth factors in the SVZ. a A 3-day injection of BBG in naïve C57BL/6 mice did not induce the expression of the general proliferation marker, Ki67, or the C cell marker EGFR. Expression of CNTF mRNA was increased, but not of FGF2, which is known to drive neuroblast formation. EGF, LIF, and IL-6, which are known to reduce neurogenesis, are increased by BBG (n=6 saline, 8 BBG). b Injection of 1 μg IL-6 directly into the striatum next to the SVZ in naïve C57BL/6 mice caused an increase in mRNA for CNTF, FGF2, LIF, and IL-6 itself in the SVZ after 24 h (n= 7 PBS, 4 IL-6). Data are mean±SEM and calculated as fold of control. *=p<0.05, **=p<0.01, ***=p<0.001. All data in Fig. 3 were obtained by qPCR