Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Dec 2.

Published in final edited form as: Free Radic Biol Med. 2012 Jun 16;53(7):10.1016/j.freeradbiomed.2012.06.008. doi: 10.1016/j.freeradbiomed.2012.06.008

Fig. 2 — Mutant Q111 cells exhibit higher basal levels of mtDNA damage and are more sensitive to H₂O₂ treatment than wild type Q7 cells. (A) Cells were treated with 200 µM H₂O₂ and DNA isolated 3 hours after treatment. Frequency of mtDNA and nDNA lesions per 10 kb per strand after normalization for changes in mtDNA abundance. n=3 independent experiments and 3 QPCR analyses. *p<0.001 versus Q7 control and **p<0.001 versus Q111 control. (B) Relative abundance of mtDNA molecules. *p<0.01 versus Q7. n=6 independent experiments. (C) Cells were treated with 200 µM H₂O₂ and cell viability was determined after 6, 12, and 24 hours of treatment using the trypan blue exclusion method. Results are expressed as % of control. *p=0.02 and **p<0.001 versus WT Q7 cells. n=2−3 independent experiments in duplicate.

Fig. 2 — Mutant Q111 cells exhibit higher basal levels of mtDNA damage and are more sensitive to H₂O₂ treatment than wild type Q7 cells. (A) Cells were treated with 200 µM H₂O₂ and DNA isolated 3 hours after treatment. Frequency of mtDNA and nDNA lesions per 10 kb per strand after normalization for changes in mtDNA abundance. n=3 independent experiments and 3 QPCR analyses. *p<0.001 versus Q7 control and **p<0.001 versus Q111 control. (B) Relative abundance of mtDNA molecules. *p<0.01 versus Q7. n=6 independent experiments. (C) Cells were treated with 200 µM H₂O₂ and cell viability was determined after 6, 12, and 24 hours of treatment using the trypan blue exclusion method. Results are expressed as % of control. *p=0.02 and **p<0.001 versus WT Q7 cells. n=2−3 independent experiments in duplicate.