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Design of a microfluidic channel for immunomagnetic capture, detection and characterization of CTCs (a). An example of distribution of captured COLO 205 cancer cells targeted with anti-EpCAM nanocarriers (b). Fluorescence and darkfield images of a captured COLO 205 cell (DAPI+/CK+/CD45−) and a white blood cell (DAPI+/CK−/CD45+); the cells were labeled using cytokeratin (CK), CD45 and DAPI stains (c).
