Figure 3. Characterization of H. pylori expressing αCgT enzymes of varying activity.

(A) Thin-layer chromatography (TLC) analysis of αCGL, αGAG, and αGPG from H. pylori expressing αCgT showing high activity (αCgT^high), low activity (αCgT^low), control activity (αCgT^cont), no activity (αCgT^Δ) and WT activity (H. pylori 26695 (WT)). Cholesteryl α-glycosides were detected by orcinol-sulfuric acid reagent after TLC. (B) Growth curve of various H. pylori clones as measured by absorbance at 600 nm. The experiment was done in duplicate and a representative result is shown. Note that a H. pylori expressing the lowest αCgT activity recombinant protein and a clone derived from Japanese clinical isolate #52 grew similarly to αCgT^low. (C) Scanning electron photomicrographs of the different H. pylori clones described above. Photomicrographs were taken at 10,000-fold magnification. Scale bar = 1 µm.