Figure 3.

Loss of BAP1 does not promote in vitro tumorigenicity in uveal melanoma cells. (a) Wound healing assays were performed on the indicated BAP1-deficient or control stable cells. (Left panel) representative pictures of Mel290 cells after stable knockdown at Day 0 (time of scratch) and Day 1. (Right panel) quantification of wound healing assays shown as percent of total area of the initial wound (b) BAP1-deficient or control Mel290 stable cells were monitored every 15mins for 16 hrs using live cell imaging. Individual cells were manually tracked and the average distance per frame (left panel) and the total distance traveled (right panel) were calculated (c) Soft agar assays performed on BAP1-deficient or control OCM1A stable cells after one week of growth. (Left panel) representative pictures of soft agar plates stained with crystal violet. (Right panel) quantification of the number of small and large colonies per plate determined using ImageJ software. * denotes P < 0.05 and ** denotes P < 0.01 based on Student’s t-test.