Figure 7.

Physical interaction between BAP1 and HCF-1 in uveal melanoma cells. Immunoprecipitations (IP) for endogenous BAP1 and HCF-1 from the combined lysates of UM cell lines. Westerns were performed on IP material and lysate supernatant that was collected after the IP (cleared lysates). α-tubulin was used as a loading control for the cleared lysate samples only. Densitometry was performed using ImageJ and showed ~75% of the total BAP1 was complexed with HCF-1. * denotes P < 0.05 and ** denotes P < 0.01 based on Student’s t-test.