Skip to main content
. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: J Biol Chem. 2005 Jul 29;280(38):10.1074/jbc.M501806200. doi: 10.1074/jbc.M501806200

FIGURE 4. Cortical granule exocytosis is inhibited by overexpression of the J-domain of csp but not by mutated J-domains.

FIGURE 4

A, PMA-evoked cortical granule exocytosis was assayed (as in Fig. 1) using control oocytes or oocytes expressing the native J-domain or mutant J-domains (either H43Q or D45A). B, immunoblot analysis of extracts of the oocytes in A used antibody that selectively binds to the amino terminus of csp. It revealed strong signal for J-domain constructs (at 12–13 kDa) only in oocytes injected with J-domain RNAs. At this exposure, the endogenous csp (at ~33 kDa) signal is very faint. The band between csp and the J-domains is nonspecific immunoreactivity.