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Oocyte extract was subjected to immunoprecipitation (IP) using csp antibody and protein A/G-agarose to capture immune complexes. Samples were resolved electrophoretically for sequential immunoblot analysis using Hsp/Hsc 70 antibody followed by csp antibody. Immunoblot signals in the lane labeled extract correspond to 25% of the oocyte extract used for immunoprecipitation.
