Table 1.
Antibody source, pre-treatment, dilution and incubation duration for tumor markers
| Primary Antibody | Clone | Species | Manufacturer | Catalog# | Pretreatment | Dilution | Diluent | Incubation Duration | Secondary Antibody | Detection Method |
|---|---|---|---|---|---|---|---|---|---|---|
| FOXP-1 | RABBIT | Sigma Aldrich, St. Louis, MO | HPA003876-100UL | VS6 *1 | 1/50 | BSA | OVN | MACH-2 Rabbit | ||
| SKP2 | 2C8D9 | MOUSE | Invitrogen, Grand Island, NY | 18-7334 | VSE *2 | 1/50 | BSA | 90 min | MACH-2 MOUSE | |
| CD44 | IM7 | MOUSE | EBIOSCIENCE, San Diego, CA | 14-0441 | VS6 *1 | 1/200 | BSA | 45′ | ENV MOUSE | |
| P53 | 1801 | MOUSE | ONCOGENE, Gibbstown, NJ | OP09-100ug | VS6 | 1/200 | BSA | 45′ | MACH-2 MOUSE | |
| CD10 | SS2/36 | MOUSE | DAKO, Carpinteria, CA | M0727 | PCE **2 | 1/100 | BSA | 45′ | ENV MOUSE | |
| BCL2 | 124 | MOUSE | DAKO, Carpinteria, CA | M0887 | VSE *2 | 1/300 | BSA | 45′ | ENV MOUSE | |
| Ki67 | MIB1 | MOUSE | DAKO, Carpinteria, CA | M7240 | VS6*1 | 1/100 | CaCl | 45′ | MACH-2 MOUSE | |
| CD30 | BERH2 | MOUSE | DAKO, Carpinteria, CA | M0751 | VS6*1 | 1/40 | BSA | 45′ | ENV MOUSE | |
| CD43 | DF-T1 | MOUSE | DAKO, Carpinteria, CA | M0786 | VS6*1 | 1/100 | BSA | 45′ | ENV MOUSE | |
| CD21 | IF8 | MOUSE | DAKO, Carpinteria, CA | M0784 | PRO K*** | 1/20 | BSA | 45′ | ENV MOUSE | |
| Cyclin D2 | DCS-3.1 + DCS-5.2 | RABBIT | Abcam, Cambridge, MA | ab3087 | VSE *2 | 1/200 | BSA | 2HR | ENV RABBIT | |
| Cyclin E | RABBIT | Abcam, Cambridge, MA | ab52189 | VSE *2 | 1/50 | BSA | OVN | ENV RABBIT | ||
| cMYC | Y69 | RABBIT | Epitomics, Burlingame, CA | 1472-1 | VSE *2 for 40 min | 1/50 | BSA | OVN | ENV RABBIT | |
| p27/Kip1 | MOUSE | BD Transduction Laboratory, San Diego, CA | 610241 | VS6*1 | 1/200 | BSA | 2hr | ENV MOUSE | ||
| Survivin | RABBIT | Novus Biological, Littleton, CO | NB500-201 | VS6 *1 | 1/50 | BSA | 45′ | ENV RABBIT | ||
| BAX | B9 | MOUSE | Santa Cruz, Santa Cruz, CA | sc-7480 | PC10**3 | 1/20 | BSA | OVN | ENV MOUSE | |
| Galectin 3 | M3/38 | RAT | Biolegend, San Diego, CA | 125402 | VS6 *1 | 1/50 | BSA | OVN | Rb anti rat | ENV RABBIT |
| BLIMP1 | 6D3 | RAT | Santa Cruz, Santa Cruz, CA | sc-47732 | VSE *2 | 1/50 | BSA | 45′ | Rb anti rat | ENV RABBIT |
| BCL6 | PG-B6P | MOUSE | DAKO, Carpinteria, CA | M7211 | VSE *2 | 1/30 | BSA | 45′ | ENV MOUSE | |
| PKC-beta 2 | Y125 | RABBIT | Abcam, Cambridge, MA | ab32026 | VSE *1 | 1/200 | BSA | OVN | ENV RABBIT | |
| MUM1 | MUM1p | MOUSE | DAKO, Carpinteria, CA | M7259 | PCE **2 | 1/100 | BSA | 45′ | ENV MOUSE | |
| LMO2 | SP51 | RABBIT | Spring Bioscience, Pleasanton, CA | M3510 | VSE *2 | 1/500 | BSA | 45′ | Novolink | |
| MMP9 | RABBIT | DAKO, Carpinteria, CA | A0150 | PC10 **3 | 1/100 | BSA | 60 min | CSA Rabbit link | CSA |
*
Vegetable steamer, 95°C for 25 min.
**
Pressure cooker, pressure cooker at 115°C for 2–3min.
***
Proteinase K, at 37°C for 10 min.
1
0.01M sodium citrate buffer, pH = 6.00.
2
0.001M EDTA, pH = 8.0.
3
0.05M Tris pH= 10.00.