Effect of MLA on phospho-active forms of ERK1/2, Akt, mTOR, and m-SR in transfected 1321N1 cells. 1321N1 Cells were transfected with siRNA against ERK1/2, Akt and mTOR, treated with MLA and processed for Western blot analysis. A. Signals associated with phosphorylated active and total forms of mTOR, AKT and ERK1/2. B. Bars represent pmTOR after quantification in control and transfected 1321N1 cells before and after treatment with MLA. C. Bars represent pAkt after quantification in control and transfected 1321N1 cells before and after treatment with MLA. D. Bars represent the pERK1/2 after quantification in control and transfected 1321N1 cells before and after treatment with MLA. E. Relative m-SR levels after quantification and normalization with β-actin are shown in bars. Data represents the average ± SD of three independent experiments. * and ***, P< 0.05 and 0.001 as compared with the control cells.