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. 2013 Sep 5;10:52. doi: 10.1186/1742-9994-10-52

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Copyright © 2013 Döring et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phylogenetic analysis of the cloned opsin sequences. The Helobdella opsins cluster well supported (96%) within the rhabdomeric opsins. They form a clade with the r-opsins from Platynereis dumerilii and Capitella teleta. The different leech opsins are more closely related to each other than separated according to the genetic strain. The tree was calculated using RAxML with 1000 puzzling steps for the bootstrap values (given in percentage). Red: annelid rhabdomeric opsins. Yellow: remaining protostome rhabdomeric opsins. Blue: non-rhabdomeric opsin families. White: Outgroup.