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. 2013 Dec 2;8(12):e80100. doi: 10.1371/journal.pone.0080100

Figure 2. MOR256-17 overexpression in S180 cells induces cell adhesion.

Figure 2

(A) Scheme of the FLAGRho-tagged OR shows the attachment of the FLAG tag and the Rho sequence at the extracellular (extra.) N-terminus of MOR256-17. (B) Combination of endogenous GFP fluorescence and anti-FLAG-immunostaining under fixed or live conditions, for cells transfected with pCAGGS-FLAGRhoMOR256-17-iresGFP ± RTP1S (B1–B4), pCAGGS-FLAGRhoα7-5HT3-iresGFP+RTP1S (B5–B6) or pCAGGS-iresGFP (CTRL) (B7–B8). Under live conditions, plasma membrane labeling for FLAGRhoMOR256-17 is visible only when cells are co-transfected with RTP1S (B4). Scale bar = 20 µm. (C) Percentage of adherent doublets of MOR256-17-expressing cells compared to CTRL or α7-5HT3-expressing cells. Significant number of adherent doublets is measured for MOR256-17 vs. MOR256-17 doublets, when compared to CTRL vs. CTRL or α7-5HT3 vs. α7-5HT3 doublets. ** p<0.01.