Table 1.
Characteristics of erythroid-specific or putative erythroid-specific DHSs of ten human KLF genes
|
KLF |
DHS |
Cs |
FAIRE |
H3K4me1 |
H3K27ac |
GATA-1 |
NF-E2 |
minP |
KLF-P |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| K562 | K562 | K562 | HeLa | HEK293 | |||||||
|
KLF1 |
I |
Y |
Y |
Y |
|
|
|
|
|
|
|
| II |
Y |
Y |
Y* |
Y* |
Y |
|
Y |
Y |
|
|
|
| III |
Y |
|
Y* |
Y* |
Y |
|
Y |
Y |
|
|
|
| IV |
Y |
Y |
|
Y* |
Y |
|
Y |
Y |
|
|
|
| V |
|
Y |
Y* |
Y* |
|
Y |
Y |
Y |
|
|
|
|
KLF2 |
I |
Y |
Y |
Y |
Y |
Y |
|
Y |
Y |
|
Y |
|
KLF3 |
I |
Y |
Y |
Y |
Y* |
|
|
|
Y |
Y |
Y |
| II |
Y |
Y |
Y |
Y* |
|
|
|
|
|
Y |
|
| III |
Y |
Y |
Y |
Y |
|
|
Y |
Y |
|
Y |
|
|
KLF6 |
I |
Y |
Y |
Y |
Y |
|
|
Y |
|
|
|
| II |
|
|
Y |
Y |
|
|
|
Y |
|
|
|
| III |
Y |
|
Y |
Y* |
|
|
|
Y |
|
|
|
| IV |
Y |
|
Y |
Y* |
|
|
Y |
Y |
|
|
|
|
KLF9 |
I |
|
Y |
Y* |
Y* |
Y |
|
Y |
Y |
|
|
| II |
|
Y |
Y |
|
Y |
|
Y |
Y |
|
|
|
|
KLF10 |
I |
|
|
Y* |
|
|
|
Y |
|
|
|
|
KLF11 |
I |
Y |
Y |
Y |
Y* |
|
|
Y |
Y |
Y |
|
|
KLF13 |
I |
|
|
Y* |
|
|
|
|
|
|
|
| II |
|
|
Y* |
|
|
|
Y |
|
|
|
|
| III |
|
|
Y |
|
|
|
|
Y |
|
|
|
|
KLF16 |
I |
|
Y |
Y* |
Y* |
|
|
Y |
|
|
|
| II |
Y |
Y |
Y |
Y |
|
|
|
|
|
|
|
| KLF17 | I | Y | Y | Y* | Y* | Y | Y | Y | |||
DHS conservation (Cs) was observed across 32 placental mammals, including humans, chimps, mice, and rabbits. The chromatin accessibility using FAIRE, enhancer-associated histone modifications (H3K4me1 and H3K27ac), and TFBSs for erythroid GATA-1 and NF-E2 on DHSs in erythroid K562 cells were extracted from UCSC Genome Browser. The enhancers characterized from the erythroid-specific or putative erythroid-specific DHSs were summarized in the last four columns. Y: yes. *: this histone modification is K562 specific or putative K562 specific.