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. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: Nat Methods. 2012 Mar 11;9(4):391–395. doi: 10.1038/nmeth.1929

Fig. 2. Refining expression patterns in VA motor neurons with a NOT gate.

Fig. 2

(a) Schematic diagram of VA and DA motor neurons in the posterior region of the VNC. DA neurons extend axonal commissures (magenta arrowheads) to the dorsal nerve cord. The unc-4 promoter is expressed in VA and DA neurons while the unc-4c promoter is only expressed in DA neurons. (b-d) The micrographs show the same region as schematically depicted in (a) in an early L4 larva expressing the indicated transgenes. Magenta shows mCherry expression and green shows GFP expression. White arrowheads indicate the commissures of DA neurons. The asterisk denotes ectopic gut fluorescence caused by SL2∷mCherry cassette. Scale bar, 20 μm.