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. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: Nat Methods. 2012 Mar 11;9(4):391–395. doi: 10.1038/nmeth.1929

Fig. 4. The Q system functions effectively with single copy transgene.

Fig. 4

(a) Schematic diagram of DA9 and VA12 neurons in the tail region. The mig-13 promoter expresses in DA9 (in magenta) and VA12 (in green) neurons while the unc-4c promoter expresses in the DA9 neuron. The magenta arrowhead indicates the commissure of DA9. (b-m) The images show the same region as schematically depicted in (a) in transgenic worms (L4 larvae) containing the indicated transgenes. GFP expression is shown green and mCherry expression is shown in magenta. Si, single insertion. The white arrowheads indicate the commissures of DA9. Scale bar, 20 μm. (n) Quantification of labeling efficiency in (b-m). Late L3 or early L4 stage larvae were scored (n>100 for each strain). Animals are divided into 3 categories: None (neither DA9 nor VA12 neurons are labeled by GFP), DA9+VA12 (both neurons are labeled), VA12 only (only VA12 neuron is labeled).