(A-H) GFP::Utrophin-CH pattern in DA9 of wildtype (A, B), plx-1 mutants (C, D), smp-1;smp-2 double mutants (E, F) and let-60(n1046gf) mutants (G, H), either GFP::Utrophin-CH signal alone (A, C, E and G), or overlaid with mCherry::RAB-3 (B, D, F and H). (I) Quantification of Utrophin-CH domains in the dorsal axon. The length of the Utrophin-CH domain from the most posterior-dorsal axon (indicated with white arrowheads) to the anterior edge (indicated with yellow arrows) was measured. Triple asterisks; p<0.001. Double asterisks; p<0.01, Single asterisk; p<0.05 (ANOVA/Tukey-HSD). Error bars; standard error of mean. (J) GFP::Utrophin-CH and mCherry::RAB-3 double labeling in unc-104 mutants. Note that the mCherry::RAB-3 signal is completely absent in the dorsal axon. Scale bar, 20μm.