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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Arch Insect Biochem Physiol. 2013 May 23;83(4):10.1002/arch.21100. doi: 10.1002/arch.21100

Fig. 3.

Fig. 3

Analysis of the purity of Hovi-mEH1 microsomal preparations by SDS-PAGE. In this representative example 2.4, 4.8, and 9.6 μg of the microsomal preparation from AcHovimEH1-infected High Five cells (lanes 1, 2, and 3, respectively) were separated by SDS-PAGE (10% NuPAGE Bis-Tris gel) and stained. The arrowhead indicates the migration of Hovi-mEH1 (52.1 kDa). In this example, Hovi-mEH1 was estimated to represent 11% of the total protein by image analysis using ImageJ software (W. S. Rasband, 2006, U. S. National Institutes of Health, http://rsb.info.nih.gov/ij/). The lanes marked M show the migration of protein standards (SeeBlue Plus2, Invitrogen); their estimated sizes are indicated between the panels in kDa. Lane 4 shows the migration of a microsomal preparation (17.7 μg) from control BacPAK6-infected High Five cells.