Table 1. Identification of Protein Spots on the 2D gel by LC-MS/MSa.
| Spot # | Protein Name | MW/pI | Coverage (%)b | Score (MinimumScore)c |
| A1-8 | GFAP: Glial fibrillary acidic protein | 50/5.4 | 38 | 302 (32) |
| VIME: Vimentin | 54/5.1 | 10 | 50 (32) | |
| B1-6 | GFAP: Glial fibrillary acidic protein | 60/4.1 | 20 | 538 (31) |
| NFL: Neurofilament light polypeptide | 61/4.6 | 5 | 113 (31) | |
| VIME: Vimentin | 54/5.1 | 30 | 43 (31) | |
| S1-9 | GFAP: Glial fibrillary acidic protein | 60/4.1 | 10 | 538 (36) |
| NFL: Neurofilament light polypeptide | 61/4.6 | 5 | 113 (36) |
a
The differentially expressed protein spots on the 2-D gels of ALS were excised and proteins identified by LC-MS/MS.
b
The length of identified peptide fragments divided by the length of the protein.
c
Mascot algorithm score; the minimum score is required to have a statistic significance (p<0.05).