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Extent of apoptosis in cultures of TK6 and WTK1 cells 23 h and 47 h following EdU pulse labeling. Exponentially grown TK6 and WTK1 cells were exposed for 1 h to 20 μM EdU, rinsed and subsequently incubated for 23 h or 47 h. Apoptotic cells were discerned as having activated (cleaved) caspase-3 (above the dashed broken line) and/or decreased (“sub-G1”) DNA content (left to the dashed vertical line) (37,38,45). Their percent is shown in the respective panels.
