Figure 6. Transcriptional responses of catA, dprA, and dprB to osmotick shock and fungicide.

(A and B) WT, ΔsakA, ΔsskA, and ΔnikA were grown in liquid YGMM for 18 h at 37°C. Then, sorbitol (A) or fludioxonil (B) was added (1 M or 10 µg/mL final concentrations, respectively). As a control, an equivalent volume of water or DMSO was added to the cultures (indicated as Control). After 15 min, the mycelium was harvested, and the RNA was extracted. cDNAs was synthesized and used for real-time RT-PCR analysis. Relative expression ratios were calculated relative to the WT control. Error bars represent the standard deviations based on three replicates. P-values were calculated by the Student’s t-test: *P<0.005; **P<0.001, compared to the Control in any strain. (C and D) WT and ΔnikA were grown in liquid YGMM for 18 h at 37°C. Then, different concentrations of sorbitol (0.25, 0.5, and 1 M final concentrations) (C) or fludioxonil (0.1, 1, and 10 µg/mL final concentrations) (D) were added. After 15 min, the mycelium was harvested, and the RNA was extracted. cDNAs was synthesized and used for real-time RT-PCR analysis. Relative expression ratios were calculated relative to WT. Error bars represent the standard deviations based on three replicates. *statistically significant difference relative to the Control in any strain (P<0.05). P-values were calculated by student’s t-test followed by Bonferroni method.