Figure 1. Study on stability and bioavailability of Δ¹²-PGJ₃.

(A–C) Δ¹²-PGJ₃ (0.5 µg/mL) was incubated in artificial gastric and intestinal juice (with or without pancreatin) for various time intervals and temperatures (4°C (A), 25°C (B), 37°C (C) and estimated for % of Δ¹²-PGJ₃ remaining by HPLC. Significant differences between concentrations Δ¹²-PGJ₃ at different time points as compared to t = 0 are indicated by *p≤0.05; **p≤0.01; ***p≤0.001 respectively. Data shown are the mean ± SEM (n = 3). (D) Time course analysis for bioavailability of Δ¹²-PGJ₃ in mouse plasma. Δ¹²-PGJ₃ (0.025 mg/kg body weight) was injected intraperitoneally into C57BL/6 mice. The concentration of Δ¹²-PGJ₃ in plasma was measured at indicated times post injection by LC-MS/MS. The chemical structure of Δ¹²-PGJ₃ is shown in the inset of panel B. All data shown are mean ± SEM (n = 3) and statistical significance represented as *- p≤0.05; **- p≤0.01; ***- p≤0.001 respectively.